Produces longer, thinner, and more flexible tips.
Insert a clean glass capillary tube through the upper slider, down through the center of the heater coil, and into the lower slider.
The Narishige PC-10 utilizes a gravity-driven vertical pulling mechanism combined with a dual-stage heating element. This design allows users to pull pipettes with long, fine tips suitable for patch-clamping, intracellular recording, microinjection, and microdissection. Key Specifications Vertical, gravity-driven dual weights. Heating Element: Nichrome heater coil. narishige pc10 manual new
Precision-machined tracks that allow the upper and lower clamps to move vertically.
A key feature that set the PC-10 apart was its two pulling modes: a single-pull and a double-pull. The single-pull mode pulls the glass in one continuous stretch, ideal for longer, thinner needles. The double-pull mode, however, is where the PC-10’s versatility shines. It allows the heating and pulling parameters to be changed mid-process, enabling researchers to produce both long, thin needles for microinjection and robust microelectrodes for patch clamping on the same device. To adjust the pulling tension, the unit comes with four weights (two light and two heavy) that can be attached or removed to fine-tune the gravitational force. Produces longer, thinner, and more flexible tips
: Users have reported variability in tip size and resistance, requiring frequent manual adjustments to maintain consistency.
Narishige PC-10 Glass Puller: Comprehensive Operational Manual and Setup Guide This design allows users to pull pipettes with
The Narishige PC-10 is a gravity-based, vertical puller. Its primary purpose is to produce high-quality microelectrodes for patch-clamp recording, intracellular recording, or microinjection. The two-stage pulling mechanism allows for greater control over the tip diameter and taper length, making it ideal for producing fine tips ( ) 1.2.2 . 2. Key Components and Controls
The is a classic vertical dual-stage micropipette puller that remains a laboratory workhorse due to its robust, simple design and cost-effectiveness compared to complex microprocessor-controlled models . While officially replaced by the PC-100 , "new" units or well-maintained legacy models are still highly sought after for electrophysiology and microinjection. Performance Review
That said, if you have an original (analog, two‑heater, gravity‑based puller), I can provide a comprehensive operational guide based on the original manual and common lab practices.
: Some experts suggest it is best suited for users who already understand pipette pulling mechanics due to the adjustment time required.